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I k Multimedia SampleTank 3 v3.0.1 MacOSX Win x64-R2R [oddsox]. IK Multimedia SampleTank 3 v3.0.1 MacOSX Win x64-R2R [oddsox].,. Quality. :. IK Multimedia SampleTank 3 v3.0.1 MacOSX Win x64-R2R [oddsox]Transcriptomic analysis of synovial fluid in a guinea pig model of arthritis reveals impaired purinergic signalling.
Rheumatoid arthritis (RA) is a common immune-mediated disease characterised by joint swelling, pain, and structural joint damage. As synovium is an important site of damage in RA, it has been proposed that the infiltration and retention of immune cells (such as T-cells) in the synovium triggers joint inflammation. To assess the role of extracellular nucleotides in the pathogenesis of RA, we developed a guinea pig model of arthritis using injections of streptococcal protein G. We subjected synovial fluid (SF) to global transcriptomic profiling, using Illumina’s Sentrix-6v4-1 Expression BeadChip. The microarray data were validated by real-time quantitative PCR and immunoblotting. The experiments showed differential expression of several genes and/or proteins related to purinergic signalling in the SF of arthritic guinea pigs (compared with controls), including the purinergic receptor P2X7 (P2RX7). Using immunofluorescence, we observed an increase in number of P2RX7-positive cells in the SF from arthritic animals. This study shows that the purinergic system is involved in the pathology of RA.Electron-capture negative ion-chemical ionization mass spectrometry of tri- and tetramethylammonium alkylammonium cations with sulfur-containing volatile salts.
The electron-capture negative ion-chemical ionization (ECNI) tandem mass spectrometric (MS) method was evaluated for analysis of tri- and tetramethylammonium (TMA)- and tetraethylammonium (ETA)-N-alkyl-N-alkyl-N-methylammonium (alkyl = ethyl, propyl, butyl, or hexyl) cations (TMA-, ETA-, N-methyl-,

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